The segregation of specific clones of cytotoxic lymphocytes in an in vitro primary response against influenza virus
Identifieur interne : 002644 ( Main/Exploration ); précédent : 002643; suivant : 002645The segregation of specific clones of cytotoxic lymphocytes in an in vitro primary response against influenza virus
Auteurs : Y. Komatsu [Nouvelle-Zélande] ; Y. Nawa [Nouvelle-Zélande] ; J. Marbrook [Nouvelle-Zélande]Source :
- Immunology Letters [ 0165-2478 ] ; 1982.
English descriptors
- Teeft :
- Clonal analysis, Clone, Contingency table, Dimple, Equal aliquots, Individual clones, Influenza, Influenza cells, Influenza virus, Lysis, Lyzed, Present address, Primary response, Specific clones, Specific lysis, Spleen, Spleen cells, Spontaneous release, Stimulator, Stimulator cells, Stimulator spleen cells, Target cells, Tissue culture, Total dimples, Total number, Uninfected cells.
Abstract
Abstract: CBA spleen cells have been stimulated in vitro with A/Jap influenza virus-infected CBA spleen cells to generate a ‘primary’ cytotoxic lymphocyte (CL) response. The culture conditions were devised to allow the segregation of individual clones of CL and cytotoxicity measured by the lysis of infected or non-infected L-929 cells. The specificity was assessed by splitting clones and measuring the ability of the clones to discriminate between pairs of targets. Influenza A/FMI and A/Jap strains were used. Subsets of clones were detected which could lyze either A/Jap-infected or A/FMI-infected target cells. In addition CL clones were found which lyzed uninfected L-929 cells and a fourth category were clones which could not discriminate between A/FMI- and A/Jap-infected targets.
Url:
DOI: 10.1016/0165-2478(82)90127-4
Affiliations:
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Komatsu</name><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Cell Biology, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Nawa, Y" sort="Nawa, Y" uniqKey="Nawa Y" first="Y." last="Nawa">Y. Nawa</name><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Cell Biology, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author><author><name sortKey="Marbrook, J" sort="Marbrook, J" uniqKey="Marbrook J" first="J." last="Marbrook">J. Marbrook</name><affiliation wicri:level="1"><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Cell Biology, University of Auckland, Auckland</wicri:regionArea><wicri:noRegion>Auckland</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunology Letters</title><title level="j" type="abbrev">IMLET</title><idno type="ISSN">0165-2478</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1982">1982</date><biblScope unit="volume">5</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="351">351</biblScope><biblScope unit="page" to="355">355</biblScope></imprint><idno type="ISSN">0165-2478</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-2478</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Clonal analysis</term><term>Clone</term><term>Contingency table</term><term>Dimple</term><term>Equal aliquots</term><term>Individual clones</term><term>Influenza</term><term>Influenza cells</term><term>Influenza virus</term><term>Lysis</term><term>Lyzed</term><term>Present address</term><term>Primary response</term><term>Specific clones</term><term>Specific lysis</term><term>Spleen</term><term>Spleen cells</term><term>Spontaneous release</term><term>Stimulator</term><term>Stimulator cells</term><term>Stimulator spleen cells</term><term>Target cells</term><term>Tissue culture</term><term>Total dimples</term><term>Total number</term><term>Uninfected cells</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: CBA spleen cells have been stimulated in vitro with A/Jap influenza virus-infected CBA spleen cells to generate a ‘primary’ cytotoxic lymphocyte (CL) response. The culture conditions were devised to allow the segregation of individual clones of CL and cytotoxicity measured by the lysis of infected or non-infected L-929 cells. The specificity was assessed by splitting clones and measuring the ability of the clones to discriminate between pairs of targets. Influenza A/FMI and A/Jap strains were used. Subsets of clones were detected which could lyze either A/Jap-infected or A/FMI-infected target cells. In addition CL clones were found which lyzed uninfected L-929 cells and a fourth category were clones which could not discriminate between A/FMI- and A/Jap-infected targets.</div></front></TEI><affiliations><list><country><li>Nouvelle-Zélande</li></country></list><tree><country name="Nouvelle-Zélande"><noRegion><name sortKey="Komatsu, Y" sort="Komatsu, Y" uniqKey="Komatsu Y" first="Y." last="Komatsu">Y. Komatsu</name></noRegion><name sortKey="Marbrook, J" sort="Marbrook, J" uniqKey="Marbrook J" first="J." last="Marbrook">J. Marbrook</name><name sortKey="Nawa, Y" sort="Nawa, Y" uniqKey="Nawa Y" first="Y." last="Nawa">Y. Nawa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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